Mucin gene expression and mouse middle ear epithelium. Int J Pediatr Otorhinolaryngol 2010 Aug;74(8):864-8
Date
09/18/2010Pubmed ID
20846498Pubmed Central ID
PMC3327946DOI
10.1016/j.ijporl.2010.04.014Scopus ID
2-s2.0-77953916090 (requires institutional sign-in at Scopus site) 14 CitationsAbstract
OBJECTIVES: To investigate the expression of recently identified human mucin genes in an in vitro model of cultured mouse middle ear epithelial cells (MMEEC).
METHODS: MMEEC were established, RNA was extracted and primers were designed for RT-PCR to assess for expression of mucin genes Muc1, Muc2, Muc3, Muc4, Muc5AC, Muc5B, Muc6, Muc7, Muc8, Muc9, Muc10, Muc11/12, Muc13, Muc15, Muc16, Muc17, Muc18, Muc19 and Muc20 expression.
RESULTS: Mucin genes Muc1, Muc2, Muc3, Muc4, Muc5AC, Muc5B, Muc9, Muc10, Muc13, Muc15, Muc16, Muc18, Muc19 and Muc20 were identified and expressed in MMEEC. The genes Muc6, Muc7, Muc8, Muc11/12 and Muc17 were not identified.
CONCLUSION: Many of the mucin genes that have been recently identified in human MEE and chinchilla MEE are also expressed in MMEEC. There are differences in expression, however, which may have implications in utilizing various animal models for study of middle ear physiology and pathogenesis; specifically as it relates to mucin gene expression.
Author List
Kerschner JE, Li J, Tsushiya K, Khampang PAuthor
Joseph E. Kerschner MD Provost, Executive Vice President, Dean, Professor in the School of Medicine Administration department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCells, Cultured
Ear, Middle
Epithelial Cells
Gene Expression Regulation
Humans
In Vitro Techniques
Mice
Models, Animal
Mucins
Otitis Media
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity
