Effect of cocaine, 95% oxygen and ellagic acid on the development and antioxidant status of cultured rat embryos. Toxicol Lett 1998 Mar 16;95(1):15-21
Date
07/03/1998Pubmed ID
9650642DOI
10.1016/s0378-4274(98)00010-1Scopus ID
2-s2.0-0032584003 (requires institutional sign-in at Scopus site) 9 CitationsAbstract
Prenatal exposure to cocaine has been associated with adverse developmental effects and current data suggest cocaine induced malformations are caused by ischemic-reperfusion injury. This study was undertaken to assess a new in vitro model which uses a routine rat whole embryo culture system that incorporates a change in oxygen status, and to examine the effects of altered oxygen status and pretreatment with ellagic acid (EA), an anti-oxidant, after cocaine exposure. Embryos were evaluated by determining a developmental score and by measuring tissue reduced glutathione (GSH) levels. Following re-oxygenation with 95% O2 for the last 6 h of culture, embryos treated with cocaine had reduced developmental scores and GSH levels. Embryos treated with cocaine and not re-oxygenated with 95% O2 did not have reduced developmental scores. EA blocked the effects of cocaine on developmental score and GSH level. These data support ischemia-reperfusion injury as the mechanism of cocaine developmental toxicity.
Author List
Bohn AA, Forsyth CS, Stoner GD, Reed DJ, Frank AAMESH terms used to index this publication - Major topics in bold
AnimalsAntioxidants
Cocaine
Ellagic Acid
Embryonic and Fetal Development
Female
Glutathione
Organ Culture Techniques
Oxygen
Pregnancy
Rats
Rats, Sprague-Dawley
Reperfusion Injury
Vasoconstrictor Agents