Differential expression of critical cellular genes in human lung adenocarcinomas and squamous cell carcinomas in comparison to normal lung tissues. Neoplasia 2002;4(2):141-50
Date
03/16/2002Pubmed ID
11896569Pubmed Central ID
PMC1550320DOI
10.1038/sj.neo.7900217Scopus ID
2-s2.0-0036122839 (requires institutional sign-in at Scopus site) 40 CitationsAbstract
The Atlas human cDNA expression array was used to evaluate gene expression profile changes in the genesis of human lung adenocarcinomas and squamous cell carcinomas. Gene expression changes between adenocarcinomas and squamous cell carcinomas were also analyzed. Of the 588 gene targets, 262 genes were expressed in these tissues and, of these, 45 genes were differentially expressed by at least two-fold in tumor tissues compared to corresponding normal tissues. Semiquantitative reverse-transcriptase polymerase chain reaction was used to confirm gene expression changes. Only those genes that reflected changes in >50% of the analyzed tissues were included in the final analysis. Ultimately, 26 genes were evaluated with 14 genes overexpressed and 12 genes underexpressed compared to matching normal lung tissues. Although similar expression changes were detected in adenocarcinomas and squamous cell carcinomas for most of the genes analyzed, some subtype-specific differences were also found. Genes encoding cell cycle regulators, intracellular signal transducers, cell receptor and adhesion molecules, growth factors, oncogenes, and apoptotic effectors were differentially expressed in this study. These gene expression changes may directly contribute to the initiation or progression of human lung cancer or may be secondary effects of the tumorigenesis process. Regardless, many of these differences may be useful in the diagnosis and/or treatment of this deadly disease.
Author List
McDoniels-Silvers AL, Stoner GD, Lubet RA, You MMESH terms used to index this publication - Major topics in bold
AdenocarcinomaAged
Bronchi
Carcinoma, Non-Small-Cell Lung
Carcinoma, Squamous Cell
Female
Gene Expression Profiling
Humans
JNK Mitogen-Activated Protein Kinases
Lung Neoplasms
Male
Middle Aged
Mitogen-Activated Protein Kinases
Oligonucleotide Array Sequence Analysis
Pulmonary Alveoli
RNA
Receptors, Androgen
Receptors, Interleukin-2
Reverse Transcriptase Polymerase Chain Reaction