Decreased TRH receptor mRNA activity precedes homologous downregulation: assay in oocytes. Science 1987 Dec 04;238(4832):1406-8
Date
12/04/1987Pubmed ID
2825350DOI
10.1126/science.2825350Scopus ID
2-s2.0-0023574359 (requires institutional sign-in at Scopus site) 59 CitationsAbstract
Ligand-induced decrease in cell-surface receptor number (homologous downregulation) is often due to rapid receptor internalization. Thyrotropin-releasing hormone (TRH), however, causes a slow downregulation of TRH receptors (TRH-Rs), with a half-time of approximately 12 hours, in GH3 rat pituitary cells. The mechanism of TRH-R downregulation was studied by monitoring TRH-evoked depolarizing currents in Xenopus oocytes injected with GH3 cell RNA as a bioassay for TRH-R messenger RNA (mRNA) activity. In GH3 cells, TRH caused a rapid decrease in TRH-R mRNA activity to 15 percent of control within 3 hours. Because the half-life of TRH-R mRNA activity in control cells was approximately 3 hours, the rapid decrease in mRNA activity was not due to inhibition of mRNA synthesis alone and may represent a post-transcriptional effect.
Author List
Oron Y, Straub RE, Traktman P, Gershengorn MCMESH terms used to index this publication - Major topics in bold
AnimalsFemale
Gene Expression Regulation
Membrane Potentials
Neoplasm Proteins
Oocytes
Pituitary Neoplasms
RNA, Messenger
RNA, Neoplasm
Rats
Receptors, Neurotransmitter
Receptors, Thyrotropin-Releasing Hormone
Thyrotropin-Releasing Hormone
Tumor Cells, Cultured
Xenopus laevis