Isolation and properties of Moloney murine leukemia virus mutants: use of a rapid assay for release of virion reverse transcriptase. J Virol 1981 Apr;38(1):239-48
Date
04/01/1981Pubmed ID
6165830Pubmed Central ID
PMC171145DOI
10.1128/JVI.38.1.239-248.1981Scopus ID
2-s2.0-0019507492 (requires institutional sign-in at Scopus site) 371 CitationsAbstract
A rapid assay for retroviral reverse transcriptase activity released into the culture medium by infected cells was developed. With the assay, 4,000 clonally infected cell lines could be tested in a few hours. We have adapted the assay for use as a screen for the detection of spontaneous viral mutants. Mutants of Moloney murine leukemia virus have been isolated which (i) produce a thermolabile reverse transcriptase, (ii) are temperature sensitive for release of enzyme activity, or (iii) can only productively infect cells already producing gag-related polypeptides. The assay has also been useful for the isolation of nonproducer cells infected with various replication-defective transforming viruses.
Author List
Goff S, Traktman P, Baltimore DMESH terms used to index this publication - Major topics in bold
Culture MediaMolecular Weight
Moloney murine leukemia virus
Mutation
RNA-Directed DNA Polymerase
Temperature
Viral Plaque Assay
