Assignment of a locus for mouse lung tumor susceptibility to proximal chromosome 19. Mamm Genome 1994 Dec;5(12):749-55
Date
12/01/1994Pubmed ID
7894154DOI
10.1007/BF00292007Scopus ID
2-s2.0-0028675618 (requires institutional sign-in at Scopus site) 56 CitationsAbstract
Previous studies have hypothesized that at least three genetic loci contribute to differences in pulmonary adenoma susceptibility between mouse strains A/J and C57BL/6J. One gene that may confer susceptibility to lung tumorigenesis is the Kras protooncogene. To identify other relevant loci involved in this polygenic trait, we determined tumor multiplicity in 56 randomly chosen N-ethyl-N-nitrosourea-treated (A/J x C57BL/6J) N1 x C57BL/6 backcross (AB6N2) progeny and correlated it with genotypes at 77 microsatellite markers spanning the genome. A correlation of lung tumor multiplicity phenotypes with genotypes of microsatellite markers on distal Chromosome (Chr) 6 in the Kras region (Pas1) was confirmed, and a new region on Chr 19 (designated Pas3) was identified that also contributes to susceptibility. Linkage analysis on Chr 19 with 270 AB6N2 mice localized the region flanked by D19Mit42 and D19Mit19 that is most closely associated with lung tumor susceptibility. The Pas3 locus may be an enhancer of the susceptibility locus on Chr 6.
Author List
Devereux TR, Wiseman RW, Kaplan N, Garren S, Foley JF, White CM, Anna C, Watson MA, Patel A, Jarchow SMESH terms used to index this publication - Major topics in bold
AdenomaAnimals
Chromosome Mapping
Female
Genetic Markers
Genetic Predisposition to Disease
Genetic Variation
Genotype
Lung Neoplasms
Male
Mice
Mice, Inbred C57BL
Mice, Inbred Strains