Faculty Collaboration Database

Dynamic association of gammaherpesvirus DNA with core histone during de novo lytic infection of primary cells. Virology 2011 Dec 20;421(2):167-72

Date

10/25/2011

Pubmed ID

22018782

Pubmed Central ID

PMC3210866

DOI

10.1016/j.virol.2011.09.024

Scopus ID

2-s2.0-80355146848 (requires institutional sign-in at Scopus site)   14 Citations

Abstract

Association of herpesvirus DNA with histones has important implications for lytic and latent infections; thus herpesviruses arbitrate interactions with histones to productively infect host cells. While regulation of alpha and betaherpesvirus chromatin during lytic infection has been actively investigated, very little is known about interaction of gammaherpesvirus DNA with histones upon de novo lytic infection. Murine gammaherpesvirus-68 (MHV68) is a rodent pathogen that offers a tractable system to study gammaherpesvirus lytic infection in primary cells. In this study we report that MHV68 promoter and orilyt sequences underwent dynamic association with histone H3 during de novo lytic infection of primary macrophages and fibroblasts. Similar to HSV-1, the degree of MHV68 DNA association with histone H3 was dependent on the multiplicity of infection and was further regulated by viral DNA synthesis. Our work sets a precedent for future studies of gammaherpesvirus chromatin during de novo lytic infection.

Author List

Mounce BC, Tsan FC, Kohler S, Cirillo LA, Tarakanova VL

Authors

Lisa A. Cirillo PhD Assistant Dean, Associate Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin
Vera Tarakanova PhD Professor in the Microbiology and Immunology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Cells, Cultured
Chromatin
DNA, Viral
Fibroblasts
Gene Expression Regulation, Viral
Histones
Macrophages
Mice
Mice, Inbred C57BL
Promoter Regions, Genetic
Rhadinovirus
Viral Proteins