Faculty Collaboration Database

Effect of 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine on calcium fluxes by human platelet microsomes. Biochem Biophys Res Commun 1984 Apr 30;120(2):474-80

Date

04/30/1984

Pubmed ID

6428395

DOI

10.1016/0006-291x(84)91278-6

Scopus ID

2-s2.0-0021289056 (requires institutional sign-in at Scopus site)   7 Citations

Abstract

Under conditions where optimal concentrations of arachidonic acid, phosphatidic acid, or the calcium ionophore A23187 caused release of 50-95% of calcium from preloaded platelet microsomes, basophil platelet activating factor (1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine, AGEPC) did not cause the release of calcium at concentrations as high as 2 X 10(-5) M. The failure to stimulate calcium release was not due to metabolism or inactivation of AGEPC. These results show that AGEPC is not a calcium ionophore and is unable to directly effect the release of calcium from microsomes by mechanisms other than ionophoric action. The increase in intracellular levels that occurs during AGEPC-induced platelet aggregation must be an indirect effect of the AGEPC.

Author List

White GC 2nd

Author

Gilbert C. White MD Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Arachidonic Acid
Arachidonic Acids
Biological Transport
Blood Platelets
Calcimycin
Calcium
Dose-Response Relationship, Drug
Humans
Microsomes
Phosphatidic Acids
Platelet Activating Factor