Medical College of Wisconsin
CTSICores SearchResearch InformaticsREDCap

Immunophenotypic stability of Sézary cells by flow cytometry: usefulness of flow cytometry in assessing response to and guiding alemtuzumab therapy. Am J Clin Pathol 2012 Mar;137(3):403-11 PMID: 22338052

Pubmed ID





Flow cytometry (FC) is frequently used to detect aberrant peripheral blood (PB) T cells ("Sézary cells") in patients with mycosis fungoides (MF) and Sézary syndrome (SS). However, immunophenotypic stability of MF/SS over time is not well characterized. We analyzed 141 PB samples from 9 cases (2 SS, 7 MF). At diagnosis, there were 3 to 5 immunophenotypic aberrancies per case (median, 4), including dim or absent CD2, CD3, CD4, CD5, CD7, or CD26 and bright CD45RO. Of 9 patients, 7 had a subsequent change in immunophenotype. All patients retained multiple aberrancies at follow-up (median, 3 per analysis; range, 2-6), of which 22.0% (81/369) were new. In 5 patients, a more than 99% decrease in absolute Sézary cell (ASC) counts by FC after alemtuzumab therapy or total skin electron beam radiation was associated with clinical improvement. We observed minor immunophenotypic changes over time in most patients with MF/SS; however, the Sézary clones maintain persistently aberrant immunophenotypes and seem amenable to follow-up with limited FC panels. ASC counts by FC correlated well with clinical response.

Author List

Vaughan J, Harrington AM, Hari PN, Kroft SH, Olteanu H


Parameswaran Hari MD Chief, Professor in the Medicine department at Medical College of Wisconsin
Alexandra M. Harrington MD Associate Professor in the Pathology department at Medical College of Wisconsin
Steven Howard Kroft MD Interim Chair, Professor in the Pathology department at Medical College of Wisconsin


2-s2.0-84859238276   14 Citations

MESH terms used to index this publication - Major topics in bold

Aged, 80 and over
Antibodies, Monoclonal, Humanized
Antibodies, Neoplasm
Antineoplastic Agents
Biomarkers, Tumor
Blood Cell Count
Drug Monitoring
Flow Cytometry
Middle Aged
Mycosis Fungoides
Sezary Syndrome
jenkins-FCD Prod-321 98992d628744e349846c2f62ac68f241d7e1ea70