Fli+ etsrp+ hemato-vascular progenitor cells proliferate at the lateral plate mesoderm during vasculogenesis in zebrafish. PLoS One 2011 Feb 25;6(2):e14732
Date
03/03/2011Pubmed ID
21364913Pubmed Central ID
PMC3045372DOI
10.1371/journal.pone.0014732Scopus ID
2-s2.0-79952155685 (requires institutional sign-in at Scopus site) 12 CitationsAbstract
BACKGROUND: Vasculogenesis, the de novo formation of blood vessels from precursor cells is critical for a developing embryo. However, the signals and events that dictate the formation of primary axial vessels remain poorly understood.
METHODOLOGY/PRINCIPAL FINDINGS: In this study, we use ets-related protein-1 (etsrp), which is essential for vascular development, to analyze the early stages of vasculogenesis in zebrafish. We found etsrp(+) cells of the head, trunk and tail follow distinct developmental sequences. Using a combination of genetic, molecular and chemical approaches, we demonstrate that fli(+)etsrp(+) hemato-vascular progenitors (FEVPs) are proliferating at the lateral plate mesoderm (LPM). The Shh-VEGF-Notch-Hey2 signaling pathway controls the proliferation process, and experimental modulation of single components of this pathway alters etsrp(+) cell numbers at the LPM.
CONCLUSIONS/SIGNIFICANCE: This study for the first time defines factors controlling proliferation, and cell numbers of pre-migratory FEVPs in zebrafish.
Author List
Chun CZ, Remadevi I, Schupp MO, Samant GV, Pramanik K, Wilkinson GA, Ramchandran RAuthor
Ramani Ramchandran PhD Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBlood Vessels
Cell Movement
Cell Proliferation
Embryo, Nonmammalian
Endothelium, Vascular
Gene Expression Regulation, Developmental
Head
Hematopoietic Stem Cells
Mesoderm
Models, Biological
Neovascularization, Physiologic
Proto-Oncogene Protein c-fli-1
Tail
Zebrafish
Zebrafish Proteins