Middle ear epithelial mucin production in response to interleukin 1beta exposure in vitro. Otolaryngol Head Neck Surg 2003 Jul;129(1):128-35
Date
07/19/2003Pubmed ID
12869928DOI
10.1016/S0194-59980300532-1Scopus ID
2-s2.0-0042786586 (requires institutional sign-in at Scopus site) 15 CitationsAbstract
OBJECTIVES: To investigate the role of the inflammatory cytokine interleukin-1beta (IL-1beta) in the regulation of mucin secretion by middle ear epithelia. Study design and setting Primary chinchilla middle ear epithelial cultures were established and exposed to IL-1beta in a dose- and time-dependent manner. Mucin secretion was characterized by exclusion chromatography and liquid scintillation.
RESULTS: Epithelial cultures exposed to increasing doses of IL-1beta demonstrated greater amounts of mucin secretion (P = 0.007). Additionally, cultures exposed to IL-1beta at 50 ng/mL showed increased secretion of mucin over control noticeable at 6 hours, but significant at the 15- and 24-hour time points (P <.0001 for each).
CONCLUSIONS: IL-1beta upregulates mucin secretion from cultured middle ear epithelial cells in a dose- and time-dependent manner.
SIGNIFICANCE: Elucidating the effect of specific cytokines on the regulation of mucin secretion is vital to understanding the pathophysiology of otitis media and the development of novel therapeutic strategies.
Author List
Kerschner JE, Meyer TK, Wohlfeill EAuthor
Joseph E. Kerschner MD Provost, Executive Vice President, Dean, Professor in the School of Medicine Administration department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsChinchilla
Ear, Middle
Epithelium
In Vitro Techniques
Interleukin-1
Mucins