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Rhythmic expression of Nocturnin mRNA in multiple tissues of the mouse. BMC Dev Biol 2001;1:9

Date

06/08/2001

Pubmed ID

11394964

Pubmed Central ID

PMC32249

DOI

10.1186/1471-213X-1-9

Scopus ID

2-s2.0-3042719574   80 Citations

Abstract

BACKGROUND: Nocturnin was originally identified by differential display as a circadian clock regulated gene with high expression at night in photoreceptors of the African clawed frog, Xenopus laevis. Although encoding a novel protein, the nocturnin cDNA had strong sequence similarity with a C-terminal domain of the yeast transcription factor CCR4, and with mouse and human ESTs. Since its original identification others have cloned mouse and human homologues of nocturnin/CCR4, and we have cloned a full-length cDNA from mouse retina, along with partial cDNAs from human, cow and chicken. The goal of this study was to determine the temporal pattern of nocturnin mRNA expression in multiple tissues of the mouse.

RESULTS: cDNA sequence analysis revealed a high degree of conservation among vertebrate nocturnin/CCR4 homologues along with a possible homologue in Drosophila. Northern analysis of mRNA in C3H/He and C57/Bl6 mice revealed that the mNoc gene is expressed in a broad range of tissues, with greatest abundance in liver, kidney and testis. mNoc is also expressed in multiple brain regions including suprachiasmatic nucleus and pineal gland. Furthermore, mNoc exhibits circadian rhythmicity of mRNA abundance with peak levels at the time of light offset in the retina, spleen, heart, kidney and liver.

CONCLUSION: The widespread expression and rhythmicity of mNoc mRNA parallels the widespread expression of other circadian clock genes in mammalian tissues, and suggests that nocturnin plays an important role in clock function or as a circadian clock effector.

Author List

Wang Y, Osterbur DL, Megaw PL, Tosini G, Fukuhara C, Green CB, Besharse JC

Author

Joseph C. Besharse PhD Director, Professor in the Ophthalmology and Visual Sciences department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Amino Acid Sequence
Animals
Biological Clocks
Cattle
Drosophila Proteins
Genes, Intracisternal A-Particle
Humans
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C3H
Mice, Inbred C57BL
Mice, Inbred Strains
Molecular Sequence Data
Nuclear Proteins
Organ Specificity
Protein Biosynthesis
Proteins
RNA, Messenger
Rats
Sequence Homology, Nucleic Acid
Transcription Factors
Xenopus Proteins
jenkins-FCD Prod-387 b0ced2662056320369de4e5cd5f21c218c03feb3