Medical College of Wisconsin
CTSICores SearchResearch InformaticsREDCap

Differential microstructure and physiology of brain and bone metastases in a rat breast cancer model by diffusion and dynamic contrast enhanced MRI. Clin Exp Metastasis 2012 Jan;29(1):51-62 PMID: 22042553 PMCID: PMC3233647

Pubmed ID

22042553

Abstract

Pharmacological approaches to treat breast cancer metastases in the brain have been met with limited success. In part, the impermeability of the blood brain barrier (BBB) has hindered delivery of chemotherapeutic agents to metastatic tumors in the brain. BBB-permeable chemotherapeutic drugs are being developed, and noninvasively assessing the efficacy of these agents will be important in both preclinical and clinical settings. In this regard, dynamic contrast enhanced (DCE) and diffusion weighted imaging (DWI) are magnetic resonance imaging (MRI) techniques to monitor tumor vascular permeability and cellularity, respectively. In a rat model of metastatic breast cancer, we demonstrate that brain and bone metastases develop with distinct physiological characteristics as measured with MRI. Specifically, brain metastases have limited permeability of the BBB as assessed with DCE and an increased apparent diffusion coefficient (ADC) measured with DWI compared to the surrounding brain. Microscopically, brain metastases were highly infiltrative, grew through vessel co-option, and caused extensive edema and injury to the surrounding neurons and their dendrites. By comparison, metastases situated in the leptomenengies or in the bone had high vascular permeability and significantly lower ADC values suggestive of hypercellularity. On histological examination, tumors in the bone and leptomenengies were solid masses with distinct tumor margins. The different characteristics of these tissue sites highlight the influence of the microenvironment on metastatic tumor growth. In light of these results, the suitability of DWI and DCE to evaluate the response of chemotherapeutic and anti-angiogenic agents used to treat co-opted brain metastases, respectively, remains a formidable challenge.

Author List

Budde MD, Gold E, Jordan EK, Frank JA

Author

Matthew Budde PhD Associate Professor in the Neurosurgery department at Medical College of Wisconsin




Scopus

2-s2.0-84856015107   21 Citations

MESH terms used to index this publication - Major topics in bold

Animals
Bone Neoplasms
Brain Neoplasms
Cell Line, Tumor
Contrast Media
Female
Humans
Magnetic Resonance Imaging
Mammary Neoplasms, Experimental
Neoplasm Transplantation
Rats
jenkins-FCD Prod-288 ba989beae37c4807010653a17407cd2d1f5e0810