In vitro transformation of rat esophageal epithelial cells with N-nitrosobenzylmethylamine. Carcinogenesis 1982;3(6):629-34
Date
01/01/1982Pubmed ID
7116556DOI
10.1093/carcin/3.6.629Scopus ID
2-s2.0-0020287317 (requires institutional sign-in at Scopus site) 20 CitationsAbstract
Using an explant/cell culture system, rat esophageal epithelial cells were transformed in vitro by exposure to N-nitroso-N-benzyl-N-methylamine (BMNA). Twelve esophageal explant cultures per group were exposed twice (at days 1 and 7) to 0.0, 2.5, 5.0 or 10.0 micrograms BMNA/ml of medium. After incubation for 60-90 days, epithelial cells in primary cultures treated with all three concentrations of BMNA could be subcultured and cell lines were developed. The number of primary cultures and the number of subsequently developed epithelial cell lines was carcinogen-dose-dependent. Cell lines could only be established from carcinogen treated explants. Electron microscopy revealed that the BMNA-treated cell lines contained morphological markers of esophageal epithelial cells; i.e., numerous tonofilaments and junctional complexes, even after prolonged subculture. By immunofluorescence, the cells reacted positively with antibodies prepared to mouse skin prekeratins (K1 and K2). Two cell lines (from the 5 micrograms BMNA/ml group) were able to grow in soft agar and produce palpable tumors upon injection into syngeneic recipients. These tumors possessed the histological features of squamous cell carcinomas.
Author List
Stoner GD, Babcock MS, Cothern GA, Klaunig JE, Gunning WT 3rd, Knipe SMMESH terms used to index this publication - Major topics in bold
AnimalsCarcinogens
Cell Line
Cell Transformation, Neoplastic
Dimethylnitrosamine
Epithelium
Esophagus
Male
Rats
Rats, Inbred F344