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Angiotensin II and VEGF are involved in angiogenesis induced by short-term exercise training. Am J Physiol Heart Circ Physiol 2001 Sep;281(3):H1163-9 PMID: 11514283


Results from our laboratory have suggested a pathway involving angiotensin II type 1 (AT(1)) receptors and vascular endothelial growth factor (VEGF) in angiogenesis induced by electrical stimulation. The present study investigated if similar mechanisms underlie the angiogenesis induced by short-term exercise training. Seven days before training and throughout the training period, male Sprague-Dawley rats received either captopril or losartan in their drinking water. Rats underwent a 3-day treadmill training protocol. The tibialis anterior and gastrocnemius muscles were harvested under anesthesia and lightly fixed in formalin (vessel density) or frozen in liquid nitrogen (VEGF expression). In controls, treadmill training resulted in a significant increase in vessel density in all muscles studied. However, the angiogenesis induced by exercise was completely blocked by either losartan or captopril. Western blot analysis showed that VEGF expression was increased in the exercised control group, and both losartan and captopril blocked this increase. The role of VEGF was directly confirmed using a VEGF-neutralizing antibody. These results confirm the role of angiotensin II and VEGF in angiogenesis induced by exercise.

Author List

Amaral SL, Papanek PE, Greene AS


Andrew S. Greene PhD Interim Vice Chair, Chief, Professor in the Biomedical Engineering department at Medical College of Wisconsin
Paula Papanek PhD, MPT, LAT, FACSM Associate Professor & Director of Exercise Science in the Exercise Science & Physical Therapy department at Marquette University

MESH terms used to index this publication - Major topics in bold

Angiotensin II
Antihypertensive Agents
Blood Pressure
Blotting, Western
Body Composition
Body Weight
Cell Count
Endothelial Growth Factors
Muscle, Skeletal
Neovascularization, Physiologic
Physical Conditioning, Animal
Rats, Sprague-Dawley
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors

View this publication's entry at the Pubmed website PMID: 11514283
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