T cell proliferation and cytokine secretion to T cell epitopes of Asp f 2 in ABPA patients. Clin Immunol 2001 Aug;100(2):228-35
Date
07/24/2001Pubmed ID
11465952DOI
10.1006/clim.2001.5056Scopus ID
2-s2.0-0034909982 (requires institutional sign-in at Scopus site) 26 CitationsAbstract
The pathogenesis of allergic bronchopulmonary aspergillosis (ABPA) involves specific cytokines secreted by lymphocytes in response to Aspergillus fumigatus (Af) allergens. To gain information about the lymphoproliferative response and cytokine production against a major Af allergen, Asp f 2, we studied Asp-f-2-specific T cell clones (TCCs) from ABPA patients. TCCs were stimulated with rAsp f 2, its deletion mutants, and synthetic peptides to identify the T cell epitope(s) and to understand cytokine production. PBMCs from four of five ABPA patients showed proliferation in response to Asp f 2. Three TCCs from one patient showed higher IL-5 secretion compared to IL-4 and IFN-gamma. Two TCCs from the second patient showed a mixed Th1/Th2 response, as evidenced by production of IL-4, IL-5, and IFN-gamma. An epitope from the N-terminal region of Asp f 2 induced only IL-5 secretion. High IL-5 secretion might explain the marked eosinophilia observed in ABPA patients.
Author List
Rathore VB, Johnson B, Fink JN, Kelly KJ, Greenberger PA, Kurup VPAuthor
Bryon D. Johnson PhD Adjunct Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Amino Acid SequenceAspergillosis, Allergic Bronchopulmonary
Cell Division
Cytokines
Fungal Proteins
Humans
Immunodominant Epitopes
Lymphocyte Activation
Molecular Sequence Data
T-Lymphocytes