Medical College of Wisconsin
CTSICores SearchResearch InformaticsREDCap

Donor natural killer (NK1.1+) cells do not play a role in the suppression of GVHD or in the mediation of GVL reactions after DLI. Biol Blood Marrow Transplant 2001;7(11):589-95

Date

01/05/2002

Pubmed ID

11760146

DOI

10.1053/bbmt.2001.v7.pm11760146

Scopus ID

2-s2.0-0035195863   11 Citations

Abstract

Donor regulatory T cells (CD3+ alphabetaT-cell receptor [TCR]+) derived from the repopulating host thymus have been shown to be primarily responsible for suppression of GVHD following DLI therapy in murine BMT models. However, natural killer (NK) T cells also have regulatory properties, and a role for NK T cells in suppression of GVH reactivity has not been completely excluded. NK cells may also contribute to the graft-versus-leukemia (GVL) effect associated with DLI therapy. In this study, we used a murine BMT model (C57BL/6 into AKR) to study whether depletion of donor NK cells had any impact on the suppression of GVH reactivity after DLI or on the DLI-induced GVL effect against acute T-cell leukemia. Depletion of donor NK cells was accomplished in vivo by giving DLI-treated bone marrow chimeras multiple injections of anti-NK1.1 monoclonal antibody (MoAb). The chimeras treated with anti-NK1.1 MoAb had significantly fewer splenic NK1.1 cells than nontreated chimeras, and splenocytes from anti-NK1.1-treated mice were deficient in the ability to generate lymphokine-activated lytic activity. Results presented here showed that NK-cell depletion had no effect on the suppression of GVH reactivity after DLI. When DLI-treated chimeras were challenged with an acute T-cell leukemia, NK-cell depletion had no discernible effect on GVL reactivity. These preclinical data suggest that donor NK cells do not have a significant role in the suppression of GVHD after DLI or in the mediation of GVL reactivity induced by DLI.

Author List

Johnson BD, Dagher N, Stankowski WC, Hanke CA, Truitt RL

Author

Bryon D. Johnson PhD Professor in the Medicine department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Antibodies, Monoclonal
Bone Marrow Transplantation
Graft Survival
Graft vs Host Disease
Graft vs Leukemia Effect
Killer Cells, Natural
Leukemia, Experimental
Leukocyte Transfusion
Mice
Mice, Inbred Strains
Models, Animal
Survival Rate
Time Factors
Transplantation Chimera
jenkins-FCD Prod-398 336d56a365602aa89dcc112f077233607d6a5abc