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An intramyocardial catheter for repeated in vivo sampling of interstitial fluid. J Pharmacol Toxicol Methods 2002 Mar-Apr;47(2):73-8

Date

12/03/2002

Pubmed ID

12459145

Abstract

INTRODUCTION: Coronary collateral development is an important adaptive response to chronic myocardial ischemia. Characterization of mitogenic factors responsible for collateral formation has been an elusive goal because these substances are difficult to sample from the myocardial interstitium at multiple times. We report the implantation of an exchange catheter capable of in vivo sampling of myocardial interstitial fluid in chronically instrumented dogs.

METHODS: The catheter consisted of multiple perforations within a 2-cm segment of Micro-Renathane tubing that was implanted into the left ventricular myocardium between the left anterior descending (LAD) and left circumflex coronary artery (LCCA) perfusion territories and secured to the epicardium with a Silastic disk. Dogs (n=5) underwent brief (2 min) LAD occlusions once per hour, 8 times/day, 7 days/week for 2 weeks to stimulate coronary collateral growth. Another group of dogs (n=6) without repetitive coronary occlusions served as controls. Myocardial interstitial fluid was collected daily, and mitogenic activity was evaluated by the proliferative responses of growth-arrested, cultured vascular smooth muscle and endothelial cells.

RESULTS: All dogs tolerated catheter implantation without complication. Each catheter functioned well throughout the duration of the experiment. Myocardial interstitial fluid obtained using the exchange catheter in this model of repetitive coronary occlusion produced marked proliferation of vascular smooth muscle and endothelial cells in vitro.

DISCUSSION: The exchange catheter enables chronic in vivo sampling of myocardial interstitial fluid and may facilitate identification of mitogens involved in coronary collateral development.

Author List

Tessmer JP, Pagel PS, Weihrauch D, Ludwig LM, Chilian WM, Kersten JR, Warltier DC

Authors

Paul S. Pagel MD, PhD Professor in the Anesthesiology department at Medical College of Wisconsin
Dorothee Weihrauch DVM, PhD Professor in the Anesthesiology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Cardiac Surgical Procedures
Catheters, Indwelling
Disease Models, Animal
Dogs
Extracellular Space
Female
Heart
Male
Mitogens
Myocardial Ischemia
Neovascularization, Physiologic
jenkins-FCD Prod-387 b0ced2662056320369de4e5cd5f21c218c03feb3