Faculty Collaboration Database

Effect of heart rate increase on dorsal aortic flow in the stage 24 chick embryo. Pediatr Res 1987 Oct;22(4):442-4

Date

10/01/1987

Pubmed ID

2960945

DOI

10.1203/00006450-198710000-00016

Scopus ID

2-s2.0-0023427233 (requires institutional sign-in at Scopus site)   29 Citations

Abstract

We evaluated the effect of increased heart rate on cardiac output and stroke volume in the stage 24 chick embryo (day 4 of a 21-day incubation). Blood flow was measured with a 20 MHz pulsed-Doppler flowmeter. Heart rate was increased by pacing with square wave stimuli (1 ms duration, less than 4 mA). The sinus venosus was paced from bipolar Teflon-coated silver electrodes in eight embryos and the ventricular apex was paced in three embryos. The pacing rates were at the intrinsic heart rate (P:I); 125% of intrinsic heart rate (P:125%I); and 150% of intrinsic heart rate (P:150%I). Physiologic measurements during pacing were compared to those obtained at the control intrinsic rate (I). We also evaluated the velocity profile of atrioventricular inflow and conotruncal outflow at intrinsic rate and during sinus venosus and ventricular pacing. With sinus venosus pacing, mean dorsal aortic blood flow was similar at control (1.07 +/- 0.05 mm3/s) and P:I (1.06 +/- 0.06 mm3/s) (mean +/- SEM). However, at P:125%I and P:150%I, mean dorsal aortic blood flow decreased significantly (P:125%I, 0.88 +/- 0.05 mm3/s; P:150%I, 0.67 +/- 0.07 mm3/s) (p less than 0.05). Stroke volume per beat also decreased with increasing heart rates (I, 0.41 +/- 0.02 mm3; P:I, 0.39 +/- 0.02 mm3; P:125%I, 0.28 +/- 0.02 mm3; P:150%I, 0.18 +/- 0.02 mm3) (p less than 0.05). With rapid sinus venosus pacing, the atrioventricular blood flow velocity profile showed a rate-dependent decrease in passive ventricular filling while active filling remained the same or increased slightly.(ABSTRACT TRUNCATED AT 250 WORDS)

Author List

Dunnigan A, Hu N, Benson DW Jr, Clark EB

MESH terms used to index this publication - Major topics in bold

Animals
Aorta
Blood Flow Velocity
Cardiac Output
Chick Embryo
Electric Stimulation
Heart
Heart Rate
Rheology