A maternal high-fat diet is accompanied by alterations in the fetal primate metabolome. Am J Obstet Gynecol 2009 Sep;201(3):281.e1-9
Date
09/08/2009Pubmed ID
19733280Pubmed Central ID
PMC2749563DOI
10.1016/j.ajog.2009.06.041Scopus ID
2-s2.0-69349095304 (requires institutional sign-in at Scopus site) 54 CitationsAbstract
OBJECTIVE: To characterize the serum metabolome of a primate model of in utero high-fat exposure.
STUDY DESIGN: Serum from maternal and fetal (e130) macaque monkeys exposed to either a high-fat or control diet were analyzed by gas chromatography-mass spectrometry. Multivariate data analysis was performed to reduce the generated data set. Candidate metabolites were further analyzed for significance by using the analysis of variance and comparative t tests.
RESULTS: Approximately 1300 chromatographic features were detected. Through multivariate data analysis this number was reduced to 60 possible metabolites. With the use of comparative t tests, 22 metabolites had statistical significance (P < .05) over the entire study. By virtue of maternal high-fat diet alone, fetal phenotypic differences are accompanied by altered metabolite concentrations of 7 metabolites (P < .05).
CONCLUSION: In utero high-fat diet exposure is associated with an altered fetal epigenome and parlays a characteristic modification in the fetal metabolite profile.
Author List
Cox J, Williams S, Grove K, Lane RH, Aagaard-Tillery KMMESH terms used to index this publication - Major topics in bold
AnimalsDietary Fats
Epigenesis, Genetic
Fetal Development
Fetus
Gas Chromatography-Mass Spectrometry
Macaca
Metabolome
Multivariate Analysis