Localization and quantification of glucose transporters in liver of growth-retarded fetal and neonatal rats. Am J Physiol 1999 Jan;276(1):E135-42
Date
01/14/1999Pubmed ID
9886959DOI
10.1152/ajpendo.1999.276.1.E135Scopus ID
2-s2.0-0032929066 (requires institutional sign-in at Scopus site) 56 CitationsAbstract
To determine whether altered transport of glucose into the hepatocyte may be an important factor contributing to abnormal hepatic glucose metabolism in the intrauterine growth-retarded (IUGR) fetus and newborn, we measured glucose transport (glucose uptake, GLUT protein, and mRNA) and localization of GLUT protein in liver of control (sham operated) and IUGR fetal (day 20) and postnatal (1, 4, 14, and 21 days) rats. GLUT-1 and -2 proteins were localized to the hepatocyte. Glucose uptake and GLUT-1 protein and mRNA levels were increased in IUGR fetal and neonatal liver. GLUT-2 protein and mRNA levels were low in IUGR and control fetal liver. After birth, GLUT-2 abundance did not differ from controls. Run-on experiments showed that the rate of transcription of GLUT-1 and -2 did not differ between IUGR and control rats. However, the transcription rate of GLUT-1 decreased with age, and the GLUT-2 transcription rate increased with age. These studies indicate that the metabolic and physiological factors that cause IUGR also alter glucose transporter expression in fetal liver.
Author List
Lane RH, Crawford SE, Flozak AS, Simmons RAMESH terms used to index this publication - Major topics in bold
AnimalsAnimals, Newborn
Blotting, Western
Fetal Growth Retardation
Fetus
Glucose
Glucose Transporter Type 1
Glucose Transporter Type 2
Immunohistochemistry
Liver
Monosaccharide Transport Proteins
RNA, Messenger
Rats
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
Tissue Distribution