Advances in identification of clinical yeast isolates by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 2013 May;51(5):1359-66
Date
02/22/2013Pubmed ID
23426924Pubmed Central ID
PMC3647935DOI
10.1128/JCM.03105-12Scopus ID
2-s2.0-84876705043 (requires institutional sign-in at Scopus site) 65 CitationsAbstract
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based identification is being adopted by clinical laboratories for routine identification of microorganisms. To date, the majority of studies have focused on the performance and optimization of MALDI-TOF MS for the identification of bacterial isolates. We review recent literature describing the use of MALDI-TOF MS for the routine identification of a variety of yeasts and yeast-like isolates. Specific topics include the effect of optimized or streamlined extraction methods, modified scoring thresholds, expanded reference libraries, and the possibility of conducting antifungal susceptibility testing using MALDI-TOF MS.
Author List
Buchan BW, Ledeboer NAAuthors
Blake W. Buchan PhD Professor in the Pathology department at Medical College of WisconsinNathan A. Ledeboer PhD Vice Chair, Professor in the Pathology department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
Antifungal AgentsCandida
Candidiasis
Cryptococcosis
Cryptococcus
Humans
Microbial Sensitivity Tests
Mycological Typing Techniques
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Yeasts