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Advances in identification of clinical yeast isolates by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 2013 May;51(5):1359-66

Date

02/22/2013

Pubmed ID

23426924

Pubmed Central ID

PMC3647935

DOI

10.1128/JCM.03105-12

Scopus ID

2-s2.0-84876705043 (requires institutional sign-in at Scopus site)   65 Citations

Abstract

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based identification is being adopted by clinical laboratories for routine identification of microorganisms. To date, the majority of studies have focused on the performance and optimization of MALDI-TOF MS for the identification of bacterial isolates. We review recent literature describing the use of MALDI-TOF MS for the routine identification of a variety of yeasts and yeast-like isolates. Specific topics include the effect of optimized or streamlined extraction methods, modified scoring thresholds, expanded reference libraries, and the possibility of conducting antifungal susceptibility testing using MALDI-TOF MS.

Author List

Buchan BW, Ledeboer NA

Authors

Blake W. Buchan PhD Professor in the Pathology department at Medical College of Wisconsin
Nathan A. Ledeboer PhD Vice Chair, Professor in the Pathology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Antifungal Agents
Candida
Candidiasis
Cryptococcosis
Cryptococcus
Humans
Microbial Sensitivity Tests
Mycological Typing Techniques
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Yeasts