Mirror-symmetric microtubule assembly and cell interactions drive lumen formation in the zebrafish neural rod. EMBO J 2013 Jan 09;32(1):30-44
Date
12/04/2012Pubmed ID
23202854Pubmed Central ID
PMC3545300DOI
10.1038/emboj.2012.305Scopus ID
2-s2.0-84872861885 (requires institutional sign-in at Scopus site) 52 CitationsAbstract
By analysing the cellular and subcellular events that occur in the centre of the developing zebrafish neural rod, we have uncovered a novel mechanism of cell polarisation during lumen formation. Cells from each side of the neural rod interdigitate across the tissue midline. This is necessary for localisation of apical junctional proteins to the region where cells intersect the tissue midline. Cells assemble a mirror-symmetric microtubule cytoskeleton around the tissue midline, which is necessary for the trafficking of proteins required for normal lumen formation, such as partitioning defective 3 and Rab11a to this point. This occurs in advance and is independent of the midline cell division that has been shown to have a powerful role in lumen organisation. To our knowledge, this is the first example of the initiation of apical polarisation part way along the length of a cell, rather than at a cell extremity. Although the midline division is not necessary for apical polarisation, it confers a morphogenetic advantage by efficiently eliminating cellular processes that would otherwise bridge the developing lumen.
Author List
Buckley CE, Ren X, Ward LC, Girdler GC, Araya C, Green MJ, Clark BS, Link BA, Clarke JDAuthor
Brian A. Link PhD Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBody Patterning
Carrier Proteins
Cell Communication
Cell Division
Cell Movement
Cell Polarity
Embryo, Nonmammalian
Green Fluorescent Proteins
Luminescent Agents
Microtubules
Mutation
Neural Tube
Neurulation
Nocodazole
Protein Transport
Recombinant Fusion Proteins
Tubulin Modulators
Zebrafish
Zebrafish Proteins
rab GTP-Binding Proteins
