Medical College of Wisconsin
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Light and temperature modulated staining of the rod outer segment distal tips with Lucifer yellow. Invest Ophthalmol Vis Sci 1985 May;26(5):628-35 PMID: 2581915

Pubmed ID

2581915

Abstract

External application of the dye Lucifer yellow to isolated retinas of Xenopus laevis causes a specific staining of the distal tips of the rod outer segment (ROS). Staining occurs most frequently in the distal 4 micron of the ROS and does not diffuse throughout the ROS cytosol. In contrast, damaged ROS fill with the dye and exhibit a diffuse fluorescence. Retinas from constant light-treated animals show a greater frequency of labeling when isolated in the light than when isolated in the dark after 0.5 and 3 hr. Increased frequency of distal tip staining for dark-treated animals can be achieved if animals are returned to the light. Distal tip labeling also occurs in cyclic-light maintained animals but at a much lower frequency. The frequency of distal tip staining can also be altered by temperature and metabolic poisons. Isolated retinas exposed to dye solutions kept at either 3 degrees C or containing the metabolic poisons, iodoacetate or dinitrophenol, exhibited a reduced frequency of staining. This suggests that staining is an active process involving cellular metabolism. The distal location, dimensions, and light dependence of staining suggests that labeled regions are destined for detachment as part of disc shedding. The sensitivity of distal tip staining to metabolic poisons suggests that the photoreceptor plays a role in determining the membrane domains destined for shedding.

Author List

Matsumoto B, Besharse JC

Author

Joseph C. Besharse PhD Director, Professor in the Ophthalmology and Visual Sciences department at Medical College of Wisconsin




Scopus

2-s2.0-0021842542   35 Citations

MESH terms used to index this publication - Major topics in bold

Animals
Isoquinolines
Light
Photoreceptor Cells
Staining and Labeling
Temperature
Xenopus laevis
jenkins-FCD Prod-353 9ccd8489072cb19f5b9f808bb23ed672c582f41e