Medical College of Wisconsin
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Cytochalasin D inhibits L-glutamate-induced disc shedding without altering L-glutamate-induced increase in adhesiveness. Exp Eye Res 1989 May;48(5):641-52 PMID: 2567678

Pubmed ID

2567678

Abstract

Excitatory amino acid-stimulated disc shedding is correlated with the appearance of microfilament-rich ensheathing processes of the retinal pigment epithelium (RPE) and increased apparent adhesiveness between photoreceptors and RPE in explanted eyecups of Xenopus laevis. We have compared the time course of disc shedding and increased retinal adherence during L-glutamate treatment. Increased adherence was measured on the basis of the tendency of the apical RPE domains to partition with isolated neural retinas. In medium supplemented with L-glutamate (12 mM) or kainate (100 microM), a glutamate analog, the time course of increased partitioning of melanin pigment-rich cell fragments which contain ensheathing processes differs, even though the kinetics of induced disc shedding is the same in either case. Co-treatment with cytochalasin D (5 microM) completely blocks L-glutamate-induced disc shedding, as well as formation of microfilament-rich ensheathing processes, even though it has little effect upon apparent adhesiveness. The virtually complete dissociation of the effects of L-glutamate on disc shedding from that on increased adhesiveness of photoreceptors to RPE suggests that increased retinal adherence and pseudopod formation may be unrelated causally.

Author List

Defoe DM, Matsumoto B, Besharse JC

Author

Joseph C. Besharse PhD Director, Professor in the Ophthalmology and Visual Sciences department at Medical College of Wisconsin




Scopus

2-s2.0-0024393819   5 Citations

MESH terms used to index this publication - Major topics in bold

Adhesiveness
Animals
Cytochalasin D
Cytochalasins
Glutamates
Glutamic Acid
Photoreceptor Cells
Pigment Epithelium of Eye
Xenopus laevis
jenkins-FCD Prod-353 9ccd8489072cb19f5b9f808bb23ed672c582f41e