Localized recruitment of a chromatin-remodeling activity by an activator in vivo drives transcriptional elongation. Genes Dev 2003 Jun 01;17(11):1392-401
Date
06/05/2003Pubmed ID
12782657Pubmed Central ID
PMC196071DOI
10.1101/gad.1071803Scopus ID
2-s2.0-0038623298 (requires institutional sign-in at Scopus site) 116 CitationsAbstract
To understand the role of chromatin-remodeling activities in transcription, it is necessary to understand how they interact with transcriptional activators in vivo to regulate the different steps of transcription. Human heat shock factor 1 (HSF1) stimulates both transcriptional initiation and elongation. We replaced mouse HSF1 in fibroblasts with wild-type and mutant human HSF1 constructs and characterized regulation of an endogenous mouse hsp70 gene. A mutation that diminished transcriptional initiation led to twofold reductions in hsp70 mRNA induction and recruitment of a SWI/SNF remodeling complex. In contrast, a mutation that diminished transcriptional elongation abolished induction of full-length mRNA, SWI/SNF recruitment, and chromatin remodeling, but minimally impaired initiation from the hsp70 promoter. Another remodeling factor, SNF2h, is constitutively present at the promoter irrespective of the genotype of HSF1. These data suggest that localized recruitment of SWI/SNF drives a specialized remodeling reaction necessary for the production of full-length hsp70 mRNA.
Author List
Corey LL, Weirich CS, Benjamin IJ, Kingston REAuthor
Ivor J. Benjamin MD Center Director, Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCell Line
Chromatin
Cloning, Molecular
DNA-Binding Proteins
Fibroblasts
Gene Expression Regulation
HSP70 Heat-Shock Proteins
Heat Shock Transcription Factors
Humans
Mice
Mice, Knockout
RNA, Messenger
Recombinant Proteins
Transcription Factors
Transcription, Genetic
Transfection
