Medulloblastoma cell line secretes platelet-derived growth factor. Pediatr Neurol 1989;5(6):347-52
Date
11/01/1989Pubmed ID
2604798DOI
10.1016/0887-8994(89)90047-7Scopus ID
2-s2.0-0024831455 (requires institutional sign-in at Scopus site) 8 CitationsAbstract
Medulloblastoma is the most common malignant childhood brain tumor in which aggressive growth produces recurrence in approximately 50% of appropriately treated cases and metastases along the neuraxis in 30%. To date, no studies exist concerning the production of autocrine growth factors by this brain tumor type. Malignant brain tumors in adults often produce platelet-derived growth factor (PDGF). A medulloblastoma cell line, TE-671, has been used for many years in pediatric neuro-oncologic studies. We assayed this medulloblastoma cell line for the production of PDGF. PDGF is produced by medulloblastoma cells grown in monolayer tissue culture and stimulates PDGF-sensitive 3T3 fibroblasts to incorporate tritiated thymidine in a dose-dependent fashion. This biologic activity is blocked by PDGF antibodies in a dose-dependent relationship. We postulate that PDGF produced by medulloblastoma cells plays a role in the growth of this tumor by stimulating mitogenic activity.
Author List
Whelan HT, Nelson DB, Strother D, Przybylski C, Figge G, Mamandi AMESH terms used to index this publication - Major topics in bold
HumansImmune Sera
Medulloblastoma
Platelet-Derived Growth Factor
Tumor Cells, Cultured