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Phospholipase Cgamma2 contributes to stable thrombus formation on VWF. FEBS Lett 2004 Aug 27;573(1-3):26-30

Date

08/26/2004

Pubmed ID

15327970

DOI

10.1016/j.febslet.2004.07.048

Scopus ID

2-s2.0-4344564590   13 Citations

Abstract

Though phospholipase C PLCgamma2 is known to play an important role in platelet activation by collagen and fibrinogen, its importance in GPIb-mediated platelet activation is less well understood. To better understand the role of PLCgamma2 in GPIb-mediated adhesion and thrombus formation, we examined the ability of wild-type and PLCgamma2- deficient murine platelets to spread on immobilized von Willebrand factor (VWF) under static conditions, and to attach to and form thrombi on VWF under conditions of arterial shear. While absence of PLCgamma2 had only a minimal effect on platelet adhesion to immobilized VWF, its absence impaired spreading and profoundly affected thrombus growth and stability on VWF.

Author List

Rathore V, Wang D, Newman DK, Newman PJ

Authors

Debra K. Newman PhD Investigator in the Blood Research Institute department at BloodCenter of Wisconsin
Debra K. Newman PhD Professor in the Pharmacology and Toxicology department at Medical College of Wisconsin
Demin Wang PhD Assistant Professor in the Microbiology and Immunology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Blood Platelets
Gene Deletion
Hemorheology
Mice
Phospholipase C gamma
Platelet Adhesiveness
Thrombosis
Type C Phospholipases
von Willebrand Factor
jenkins-FCD Prod-398 336d56a365602aa89dcc112f077233607d6a5abc