Medical College of Wisconsin
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Characterization of prostate cancer in needle biopsy by cathepsin B, cell proliferation and DNA ploidy. Anticancer Res 2010 Mar;30(3):719-25

Date

04/16/2010

Pubmed ID

20392989

Scopus ID

2-s2.0-77951045911   2 Citations

Abstract

BACKGROUND: Our objective was to determine localization patterns of three distinct groups of biomarkers (cathepsin B, MIB-1 and DNA ploidy) in prostate needle biopsy sections to establish localization similarities (or differences) in biopsy and retropubic prostatectomy specimens (RPs).

MATERIALS AND METHODS: Prostate needle biopsy specimens and matched RPs from 47 patients with cancer were evaluated. Biopsy and RP sections were stained with anti-cathepsin B (CB) and anti-stefin (cystatin) A (SA) and for cell proliferation and DNA ploidy. The ratio of CB to SA in stained cells was calculated for each biopsy cancer and matched benign prostatic hyperplasia (BPH) sample.

RESULTS: The geometric mean of CB to SA was 1.45 in BPH and 2.99 in cancer specimens (p=0.0001). The percentage of S-phase cells and DNA ploidy status in needle biopsy was associated with cancer volume in RP cases (p=0.03).

CONCLUSION: Our study has indicated that the ratio of CB to SA is significantly higher in prostate cancer biopsy specimens than in BPH. The percentage of S-phase cells and DNA ploidy in needle biopsies predicts cancer volume of RPs. We have shown that localization of three distinct biomarkers in biopsies reliably assesses the nature of prostate cancer in biopsy sections.

Author List

Qian J, Bostwick DG, Iczkowski KA, Betre K, Wilson MJ, LE C, Sinha AA

Author

Kenneth A. Iczkowski MD Professor in the Pathology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Aged
Biopsy, Needle
Cathepsin B
Cell Growth Processes
Cystatins
DNA, Neoplasm
Humans
Immunohistochemistry
Male
Middle Aged
Ploidies
Prostatic Neoplasms
Ubiquitin-Protein Ligases
jenkins-FCD Prod-398 336d56a365602aa89dcc112f077233607d6a5abc