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Intravoxel distribution of DWI decay rates reveals C6 glioma invasion in rat brain. Magn Reson Med 2004 Nov;52(5):994-1004 PMID: 15508160


The hypothesis was tested that the intravoxel distribution of water diffusion rates, as measured with a stretched-exponential model of diffusion-weighted imaging (DWI), is a marker of brain tumor invasion. Eight rats underwent intracerebral inoculation of C6 glioma cells. In three rats, cells were labeled with a fluorescent dye for microscopy. One rat was inoculated with a saline solution, and five more rats were imaged without inoculation as controls. Five healthy uninoculated rats were also imaged. DWI was performed 14-15 days after inoculation, with diffusion-weighting factor b = 500 to 6500 sec/mm2, and the resulting signal attenuation was fitted with the stretched-exponential model. The heterogeneity index values were significantly lower (P < 0.05) in the peritumor ROI than in normal gray matter and significantly higher than in normal white matter. The distributed diffusion coefficient values were significantly lower than in normal white matter or normal gray matter. Fluorescence microscopy confirmed the presence of tumors in the peritumor region that could be histologically distinguished from the main tumor mass. There was no change in proton density or T2-weighted images in the peritumor region, making vasogenic edema unlikely as a source of contrast. It is therefore thought that the heterogeneity parameter alpha is a marker of brain tumor invasion.

Author List

Bennett KM, Hyde JS, Rand SD, Bennett R, Krouwer HG, Rebro KJ, Schmainda KM


Scott D. Rand MD, PhD Professor in the Radiology department at Medical College of Wisconsin
Kathleen M. Schmainda PhD Professor in the Radiology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Body Water
Brain Neoplasms
Diffusion Magnetic Resonance Imaging
Image Processing, Computer-Assisted
Microscopy, Fluorescence
Models, Statistical
Neoplasm Invasiveness
Rats, Sprague-Dawley
Tumor Cells, Cultured

View this publication's entry at the Pubmed website PMID: 15508160
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