Lesion-induced increase in survival and migration of human neural progenitor cells releasing GDNF. Cell Transplant 2008;17(7):753-62
Date
12/03/2008Pubmed ID
19044202Pubmed Central ID
PMC2650839DOI
10.3727/096368908786516819Scopus ID
2-s2.0-58149198246 (requires institutional sign-in at Scopus site) 44 CitationsAbstract
The use of human neural progenitor cells (hNPC) has been proposed to provide neuronal replacement or astrocytes delivering growth factors for brain disorders such as Parkinson's and Huntington's disease. Success in such studies likely requires migration from the site of transplantation and integration into host tissue in the face of ongoing damage. In the current study, hNPC modified to release glial cell line-derived neurotrophic factor (hNPCGDNF) were transplanted into either intact or lesioned animals. GDNF release itself had no effect on the survival, migration, or differentiation of the cells. The most robust migration and survival was found using a direct lesion of striatum (Huntington's model) with indirect lesions of the dopamine system (Parkinson's model) or intact animals showing successively less migration and survival. No lesion affected differentiation patterns. We conclude that the type of brain injury dictates migration and integration of hNPC, which has important consequences when considering transplantation of these cells as a therapy for neurodegenerative diseases.
Author List
Behrstock S, Ebert AD, Klein S, Schmitt M, Moore JM, Svendsen CNAuthor
Allison D. Ebert PhD Associate Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCell Differentiation
Cell Movement
Cell Survival
Cells, Cultured
Corpus Striatum
Glial Cell Line-Derived Neurotrophic Factor
Humans
Neurodegenerative Diseases
Neurons
Stem Cell Transplantation
Stem Cells