Isolating, expanding, and infecting human and rodent fetal neural progenitor cells. Curr Protoc Stem Cell Biol 2008 Sep;Chapter 2:Unit 2D.2
Date
09/27/2008Pubmed ID
18819085DOI
10.1002/9780470151808.sc02d02s6Scopus ID
2-s2.0-60849133362 (requires institutional sign-in at Scopus site) 24 CitationsAbstract
Neural progenitor cells have tremendous utility for understanding basic developmental processes, disease modeling, and therapeutic intervention. The protocols described in this unit provide detailed information to isolate and expand human and rodent neural progenitor cells in culture for several months as floating aggregates (termed neurospheres) or plated cultures. Detailed protocols for cryopreservation, neural differentiation, exogenous gene expression using lentivirus, and transplantation into the rodent nervous system are also described.
Author List
Ebert AD, McMillan EL, Svendsen CNAuthor
Allison D. Ebert PhD Associate Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCell Differentiation
Cell Proliferation
Cell Separation
Clone Cells
Cryopreservation
Enzymes
Fetus
Humans
Lentivirus
Lentivirus Infections
Neurons
Rodentia
Spheroids, Cellular
Stem Cell Transplantation
Stem Cells
Time Factors
Transgenes