Faculty Collaboration Database

A latex particle assay for platelet-associated IgG. Transfusion 1992;32(3):235-8

Date

03/01/1992

Pubmed ID

1557805

DOI

10.1046/j.1537-2995.1992.32392213807.x

Scopus ID

2-s2.0-0026549013 (requires institutional sign-in at Scopus site)   7 Citations

Abstract

A modified, sensitive, solid-phase assay for platelet-associated IgG is reported. Direct comparisons were made between a 125I monoclonal radioimmunoassay (RIA) and the polyclonal antibody latex particle assay. In 209 simultaneous comparisons with the RIA, the sensitivity of the latex test was 91 percent, specificity was 100 percent, and overall efficiency was 97 percent. Commencing with platelet-rich plasma, the direct latex particle test for platelet-bound IgG requires 45 minutes; 90 minutes are required to crossmatch one patient with 12 donors. The advantages of the latex assay are absence of radioactivity, stability of reagents, economy, speed, specificity, and sensitivity.

Author List

Ramos RR, Curtis BR, Chaplin H

Author

Brian Curtis PhD Director in the Platelet & Neutrophil Immunology Laboratory department at BloodCenter of Wisconsin

MESH terms used to index this publication - Major topics in bold

Antibodies, Monoclonal
Blood Platelets
Humans
Immunoglobulin G
Methods
Radioimmunoassay
Sensitivity and Specificity