Impact of haplotypes of TNF in the natural course of infective endocarditis. Clin Microbiol Infect 2014 May;20(5):459-64
Date
10/30/2013Pubmed ID
24165416DOI
10.1111/1469-0691.12370Scopus ID
2-s2.0-84901597189 (requires institutional sign-in at Scopus site) 8 CitationsAbstract
Based on previous findings for the role of single nucleotide polymorphisms (SNPs) of TNF for the predisposition for bloodstream infections, this study investigates the role of these SNPs at the promoter positions -376, -308, -238 in infective endocarditis (IE). In a case-control study, 83 patients with IE and 83 controls were enrolled. Blood genotyping for the presence of G or A alleles of the three SNPs was carried out using restriction fragment length polymorphisms. Haplotypes were calculated. Patients were mostly infected by Staphylococcus aureus (32.5%) and by species of enterococci (14.3%) and streptococci (14.3%). Carriage of the minor frequency A alleles at -238 of the promoter region of TNF was greater than in controls (8.4% versus 1.2%, p 0.003). The presence of any of the three GGA/GAA/AGA haplotypes was more frequent in patients with IE (OR 8.22, 95CI% 1.8-37.4, p 0.001). After multivariate logistic regression analysis, it was found that the only factor related to fatal outcome was carriage of the wild-type GGG haplotype (OR, 3.29, 95CI%, 1.05-10.29, p 0.04). GGA, AGA and GAA haplotypes were more frequent in patients with IE than in controls, suggesting a predisposition for IE and a potential protective role against fatal outcome, as the wild-type GGG haplotype was independently related with death.
Author List
Giannitsioti E, Damoraki G, Rokkas C, Tsaganos T, Fragou A, Kannelaki S, Athanasia S, Giamarellos-Bourboulis EJMESH terms used to index this publication - Major topics in bold
AdultAged
Case-Control Studies
Endocarditis, Bacterial
Enterococcus
Female
Gram-Positive Bacterial Infections
Haplotypes
Humans
Male
Middle Aged
Polymorphism, Restriction Fragment Length
Polymorphism, Single Nucleotide
Promoter Regions, Genetic
Prospective Studies
Staphylococcus aureus
Streptococcus
Tumor Necrosis Factor-alpha