Opportunities for live cell FT-infrared imaging: macromolecule identification with 2D and 3D localization. Int J Mol Sci 2013 Nov 19;14(11):22753-81
Date
11/22/2013Pubmed ID
24256815Pubmed Central ID
PMC3856089DOI
10.3390/ijms141122753Scopus ID
2-s2.0-84887985788 (requires institutional sign-in at Scopus site) 26 CitationsAbstract
Infrared (IR) spectromicroscopy, or chemical imaging, is an evolving technique that is poised to make significant contributions in the fields of biology and medicine. Recent developments in sources, detectors, measurement techniques and speciman holders have now made diffraction-limited Fourier transform infrared (FTIR) imaging of cellular chemistry in living cells a reality. The availability of bright, broadband IR sources and large area, pixelated detectors facilitate live cell imaging, which requires rapid measurements using non-destructive probes. In this work, we review advances in the field of FTIR spectromicroscopy that have contributed to live-cell two and three-dimensional IR imaging, and discuss several key examples that highlight the utility of this technique for studying the structure and chemistry of living cells.
Author List
Mattson EC, Aboualizadeh E, Barabas ME, Stucky CL, Hirschmugl CJAuthor
Cheryl L. Stucky PhD Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Cell TrackingHumans
Imaging, Three-Dimensional
Macromolecular Substances
Spectrophotometry, Infrared
Spectroscopy, Fourier Transform Infrared
