Merocyanine 540-sensitized photoinactivation of human erythrocytes parasitized by Plasmodium falciparum. Blood 1992 Jul 01;80(1):21-4
Date
07/01/1992Pubmed ID
1611086Scopus ID
2-s2.0-0026750976 (requires institutional sign-in at Scopus site) 19 CitationsAbstract
The purpose of this study was to evaluate the photosensitizing dye merocyanine 540 (MC540) as a means for extracorporeal purging of Plasmodium falciparum-infected erythrocytes from human blood. Parasitized red blood cells bound more dye than nonparasitized cells, and exposure to MC540 and light under conditions that are relatively well tolerated by normal erythrocytes and normal pluripotent hematopoietic stem cells reduced the concentration of parasitized cells by as much as 1,000-fold. Cells parasitized by the chloroquine-sensitive HB3 clone and the chloroquine-resistant Dd2 clone of P falciparum were equally susceptible to MC540-sensitized photolysis. These data suggest the potential usefulness of MC540 in the purging of P falciparum-infected blood.
Author List
Smith OM, Dolan SA, Dvorak JA, Wellems TE, Sieber FAuthor
Fritz Sieber PhD Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsChloroquine
Drug Resistance
Erythrocytes
Humans
In Vitro Techniques
Malaria, Falciparum
Photochemotherapy
Plasmodium falciparum
Pyrimidinones