Ferric chloride-induced murine carotid arterial injury: A model of redox pathology. Redox Biol 2013;1(1):50-5
Date
01/01/2013Pubmed ID
25101237Pubmed Central ID
PMC4116643DOI
10.1016/j.redox.2012.11.001Scopus ID
2-s2.0-84878783769 (requires institutional sign-in at Scopus site) 70 CitationsAbstract
Ferric chloride (FeCl3) induced vascular injury is a widely used model of occlusive thrombosis that reports platelet activation in the context of an aseptic closed vascular system. This model is based on redox-induced endothelial cell injury, which is simple and sensitive to both anticoagulant and anti-platelets drugs. The time required for platelet aggregation to occlude blood flow gives a quantitative measure of vascular damage that is pathologically relevant to thrombotic disease. We have refined the traditional FeCl3-induced carotid artery model making the data highly reproducible with lower variation. This paper will describe our artifices and report the role of varying the oxidative damage by varying FeCl3 concentrations and exposure. To explore a maximum difference between experimental groups, adjustment of the selected FeCl3 dose and exposure duration may be necessary.
Author List
Li W, McIntyre TM, Silverstein RLAuthor
Roy L. Silverstein MD Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCarotid Artery Injuries
Chlorides
Disease Models, Animal
Ferric Compounds
Mice
Mice, Inbred C57BL
Oxidation-Reduction