Laser-Induced FLuorescence in the Detection of Esophageal Carcinoma Proc SPIE Proc. SPIE 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging, 14 (January 4, 1995); doi:10.1117/12.198731
Date
01/04/1995Abstract
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Proceedings Article
Laser-induced fluorescence in the detection of esophageal carcinoma
Kenneth K. Wang ; Kumar Gutta ; Mark A. Laukka ; John Densmore
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Proc. SPIE 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging, 14 (January 4, 1995); doi:10.1117/12.198731
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From Conference Volume 2324
Optical Biopsy and Fluorescence Spectroscopy and Imaging
Rinaldo Cubeddu; Renato Marchesini; Serge R. Mordon; Katarina Svanberg; Herbert H. Rinneberg; Georges A. Wagnieres
Lille, France | September 06, 1994
Abstract
abstract
Laser induced fluorescence (LIF) is a technique which can perform an 'optical biopsy' of gastrointestinal mucosa. LIF was performed in resected specimens using a pulsed N2-laser coupled fiberoptically to a probe. Fluorescence was measured using a 0.2 meter spectroscope with an intensified photodiode array. Measurements were made on fresh (<30 minutes after resection) esophageal specimens containing normal mucosa, Barrett's esophagus, and adenocarcinoma. Each tissue section was examined using an optical probe consisting of a central fiber for delivering the excitation energy and a 6 fiber bundle surrounding the central fiber for detection of the fluorescence. An excitation wavelength of 337 nm was used which generated 3-ns pulses while fluorescence intensities were acquired from 300-800 nm. Spectra were obtained from each section in a standardized fashion and background spectra subtracted. Fluorescence readings were taken from 54 normal esophageal sections and 32 sections of adenocarcinoma. A fluorescence index obtained from the tumor sections was 0.68+/- 0.01 compared with 0.51+/- 0.01 for the normal sections (p<0.001). Using a discriminant value of 0.65, this technique had a sensitivity of 81% and a specificity of 100% for detection of malignant tissue. The positive predictive value was 100% and the negative predictive value was 90% for an overall accuracy of 93%. LIF is a promising technique which has the capability of distinguishing normal versus malignant tissue in the esophagus with good accuracy.
Author List
Wang KK, Gutta K, Laukka MA, Densmore JAuthor
John C. Densmore MD Professor in the Surgery department at Medical College of WisconsinView Online