Fully adaptive FEM based fluorescence optical tomography from time-dependent measurements with area illumination and detection. Med Phys 2006 May;33(5):1299-310
Date
06/07/2006Pubmed ID
16752565DOI
10.1118/1.2190330Scopus ID
2-s2.0-33646464237 (requires institutional sign-in at Scopus site) 48 CitationsAbstract
Using an area-illumination and area-detection scheme, we acquire fluorescence frequency domain measurements from a tissue phantom with an embedded fluorescent target and obtain tomographic reconstructions of the interior fluorescence absorption map with an adaptive finite element based scheme. The tissue phantom consisted of a clear acrylic cubic box (512 ml) filled with 1% Liposyn solution, while the fluorescent targets were 5 mm diameter glass bulbs filled with 1 microM Indocyanine Green dye solution in 1% Liposyn. Frequency domain area illumination and detection employed a planar excitation source using an expanded intensity modulated (100 MHz) 785 nm diode laser light and a gain modulated image intensified charge coupled device camera, respectively. The excitation pattern was characterized by isolating the singly scattered component with cross polarizers and was input into a dual adaptive finite element-based scheme for three dimensional reconstructions of fluorescent targets embedded beneath the phantom surface. Adaptive mesh refinement techniques allowed efficient simulation of the incident excitation light and the reconstruction of fluorescent targets buried at the depths of 1 and 2 cm. The results demonstrate the first clinically relevant noncontact fluorescence tomography with adaptive finite element methods.
Author List
Joshi A, Bangerth W, Hwang K, Rasmussen JC, Sevick-Muraca EMAuthor
Amit Joshi PhD Professor in the Biomedical Engineering department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AlgorithmsComputer Simulation
Finite Element Analysis
Image Enhancement
Image Interpretation, Computer-Assisted
Imaging, Three-Dimensional
Lighting
Microscopy, Fluorescence
Models, Biological
Phantoms, Imaging
Reproducibility of Results
Sensitivity and Specificity
Tomography, Optical