Evidence for the existence of an acetone soluble renin inhibiting factor in normal human plasma. Circ Res 1975 Jun;36(6 Suppl 1):17-27
Date
06/01/1975Pubmed ID
1132076DOI
10.1161/01.res.36.6.17Scopus ID
2-s2.0-0016746366 (requires institutional sign-in at Scopus site) 20 CitationsAbstract
After addition of exogenous human renin, the in vitro rate of angiotensin I generation is faster in plasma of patients with chronic renal insufficiency and, to a lesser extent, in plasma of patients with essential hypertension than in plasma from normotensive control subjects. The increased reactivity of renin in hypertensive and uremic plasma is not related to differences of endogenous renin activity, angiotensinase activity, renin substrate concentration, or substrate reactivity. Addition of normal, hypertensive, and uremic plasma to a human renin-sheep renin substrate system inhibited the rate of angiotensin generation, although significantly less inhibition was observed with uremic plasma. The reactivity of renin increased in normal plasma but not in uremic plasma after treatment with 95% acetone. After acetone extraction renin reactivity in normal and plasma inhibited the rate of angiotensin generation in a renin-renin substrate system. Less inhibition occurred with the acetone extract from a pool of uremic plasma. These results provide evidence for the existence of a naturally occurring acetone soluble renin inhibiting factor in normal and uremic plasma. The increased reactivity of renin in uremic plasma may be related to a deficiency of this factor.
Author List
Kotchen TA, Talwalkar RT, Kotchen JM, Miller MC, Welch WJMESH terms used to index this publication - Major topics in bold
AcetoneAngiotensin II
Angiotensinogen
Humans
Hypertension
In Vitro Techniques
Kinetics
Radioimmunoassay
Renin
Solubility
Time Factors
Uremia
