Characterization of vascular endothelial growth factor receptors on the endothelial cell surface during hypoxia using whole cell binding arrays. Anal Biochem 2007 Oct 15;369(2):241-7
Date
07/13/2007Pubmed ID
17624292Pubmed Central ID
PMC2706495DOI
10.1016/j.ab.2007.06.010Scopus ID
2-s2.0-34548456009 (requires institutional sign-in at Scopus site) 11 CitationsAbstract
Angiogenesis plays a central role in a variety of important biological processes such as reproduction, tissue development, and wound healing, as well as being critical to tumor formation in cancer. The development of chromosomal substitution (consomic) rat strains has permitted the chromosomal localization of genetic factors critical to angiogenesis, but many questions remain as to the mechanisms involved. Here we utilize a novel cell capture assay to assess changes in the functional expression of vascular endothelial growth factor (VEGF) receptors on the surface of vascular endothelial cells isolated from rat strains that are normal or impaired in angiogenesis. We show that functional VEGF receptor expression is increased under hypoxic conditions in rat strains that exhibit normal angiogenesis but not in a strain impaired in angiogenesis. This result implicates the dysregulation of VEGF receptor expression levels on the endothelial cell surface as a key factor in impaired angiogenesis.
Author List
Lee JE, Didier DN, Lockett MR, Scalf M, Greene AS, Olivier M, Smith LMMESH terms used to index this publication - Major topics in bold
Angiogenesis Inducing AgentsAnimals
Binding Sites
Cell Hypoxia
Cell Membrane
Endothelial Cells
Gene Expression Regulation
Microscopy
Rats
Rats, Inbred Strains
Receptors, Vascular Endothelial Growth Factor
Reproducibility of Results