Medical College of Wisconsin
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DIRECTIONAL OPTICAL COHERENCE TOMOGRAPHY PROVIDES ACCURATE OUTER NUCLEAR LAYER AND HENLE FIBER LAYER MEASUREMENTS. Retina 2015 Aug;35(8):1511-20 PMID: 25829348 PMCID: PMC4514548

Pubmed ID

25829348

DOI

10.1097/IAE.0000000000000527

Abstract

PURPOSE: The outer nuclear layer (ONL) contains photoreceptor nuclei, and its thickness is an important biomarker for retinal degenerations. Accurate ONL thickness measurements are obscured in standard optical coherence tomography (OCT) images because of Henle fiber layer (HFL). Improved differentiation of the ONL and HFL boundary is made possible by using directional OCT, a method that purposefully varies the pupil entrance position of the OCT beam.

METHODS: Fifty-seven normal eyes were imaged using multiple pupil entry positions with a commercial spectral domain OCT system. Cross-sectional image sets were registered to each other and segmented at the top of HFL, the border of HFL and the ONL and at the external limiting membrane. Thicknesses of the ONL and HFL were measured and analyzed.

RESULTS: The true ONL and HFL thicknesses varied substantially by eccentricity and between individuals. The true macular ONL thickness comprised an average of 54.6% of measurements that also included HFL. The ONL and HFL thicknesses at specific retinal eccentricities were poorly correlated.

CONCLUSION: Accurate ONL and HFL thickness measurements are made possible by the optical contrast of directional OCT. Distinguishing these individual layers can improve clinical trial endpoints and assessment of disease progression.

Author List

Lujan BJ, Roorda A, Croskrey JA, Dubis AM, Cooper RF, Bayabo JK, Duncan JL, Antony BJ, Carroll J

Author

Joseph J. Carroll PhD Director, Professor in the Ophthalmology and Visual Sciences department at Medical College of Wisconsin




Scopus

2-s2.0-84938819727   36 Citations

MESH terms used to index this publication - Major topics in bold

Adolescent
Adult
Cell Nucleus
Ependymoglial Cells
Female
Fourier Analysis
Healthy Volunteers
Humans
Male
Nerve Fibers
Prospective Studies
Retinal Neurons
Tomography, Optical Coherence
Young Adult
jenkins-FCD Prod-353 9ccd8489072cb19f5b9f808bb23ed672c582f41e