Faculty Collaboration Database

Restoring susceptibility induced MRI signal loss in rat brain at 9.4 T: A step towards whole brain functional connectivity imaging. PLoS One 2015;10(4):e0119450

Date

04/07/2015

Pubmed ID

25844644

Pubmed Central ID

PMC4386820

DOI

10.1371/journal.pone.0119450

Scopus ID

2-s2.0-84926645905 (requires institutional sign-in at Scopus site)   13 Citations

Abstract

The aural cavity magnetic susceptibility artifact leads to significant echo planar imaging (EPI) signal dropout in rat deep brain that limits acquisition of functional connectivity fcMRI data. In this study, we provide a method that recovers much of the EPI signal in deep brain. Needle puncture introduction of a liquid-phase fluorocarbon into the middle ear allows acquisition of rat fcMRI data without signal dropout. We demonstrate that with seeds chosen from previously unavailable areas, including the amygdala and the insular cortex, we are able to acquire large scale networks, including the limbic system. This tool allows EPI-based neuroscience and pharmaceutical research in rat brain using fcMRI that was previously not feasible.

Author List

Li R, Liu X, Sidabras JW, Paulson ES, Jesmanowicz A, Nencka AS, Hudetz AG, Hyde JS

Authors

Andrew S. Nencka PhD Director, Associate Professor in the Radiology department at Medical College of Wisconsin
Eric Paulson PhD Chief, Professor in the Radiation Oncology department at Medical College of Wisconsin
Jason W. Sidabras PhD Assistant Professor in the Biophysics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Brain
Brain Mapping
Ear, Middle
Echo-Planar Imaging
Fluorocarbons
Image Processing, Computer-Assisted
Radiography
Rats