Spatial distribution of intraflagellar transport proteins in vertebrate photoreceptors. Vision Res 2008 Feb;48(3):413-23
Date
10/13/2007Pubmed ID
17931679Pubmed Central ID
PMC2276311DOI
10.1016/j.visres.2007.08.022Scopus ID
2-s2.0-38749125109 (requires institutional sign-in at Scopus site) 29 CitationsAbstract
Intraflagellar transport (IFT) of a approximately 17S particle containing at least 16 distinct polypeptides is required for the assembly and maintenance of cilia and flagella. Although both genetic and biochemical evidence suggest a role for IFT in vertebrate photoreceptors, the spatial distribution of IFT proteins within photoreceptors remains poorly defined. We have evaluated the distribution of 4 IFT proteins using a combination of immunocytochemistry and rod-specific overexpression of GFP tagged IFT proteins. Endogenous IFT proteins are most highly concentrated within the inner segment, around the basal body, and within the outer segment IFT proteins are localized in discrete particles along the entire length of the axoneme. IFT52-GFP and IFT57-GFP mimicked this pattern in transgenic Xenopus.
Author List
Luby-Phelps K, Fogerty J, Baker SA, Pazour GJ, Besharse JCMESH terms used to index this publication - Major topics in bold
AnimalsAnimals, Genetically Modified
Carrier Proteins
Embryo, Nonmammalian
Eye Proteins
Mice
Photoreceptor Cells, Vertebrate
Retina
Retinal Rod Photoreceptor Cells
Ultrasonography
Xenopus