Comparative metabolism and macromolecular binding of benzo[a]pyrene in explant cultures of human bladder, skin, bronchus and esophagus from eight individuals. Cancer Lett 1983 Feb;18(1):11-9
Date
02/01/1983Pubmed ID
6825070DOI
10.1016/0304-3835(83)90112-xScopus ID
2-s2.0-0021067426 (requires institutional sign-in at Scopus site) 12 CitationsAbstract
Benzo[a]pyrene (B[a]P) metabolism and macromolecular binding have been studied in explants from 4 tissues, bladder, skin, bronchus and esophagus from 8 donors sampled within 4 h after death. Explants were incubated with [3H]benzo[a]pyrene for 24 h, then the metabolites extracted and analyzed by high-pressure liquid chromatography. Fibroblasts were grown from explants from 2 patients and also incubated with B[a]P. Metabolite profiles were qualitatively the same for explants and fibroblasts with similar product ratios, although fibroblasts were less active in B[a]P metabolism. DNA binding studies showed a broad variance between patients and tissues with the relative distribution being widest in bladder, followed by skin, bronchus and esophagus, respectively.
Author List
Selkirk JK, Nikbakht A, Stoner GDMESH terms used to index this publication - Major topics in bold
BenzopyrenesBronchi
Culture Techniques
Esophagus
Fibroblasts
Humans
Skin
Tissue Distribution
Urinary Bladder