Faculty Collaboration Database

Inhibited mitochondrial respiration by amobarbital during cardiac ischaemia improves redox state and reduces matrix Ca2+ overload and ROS release. Cardiovasc Res 2008 Jan 15;77(2):406-15

Date

09/29/2007

Pubmed ID

17900548

DOI

10.1016/j.cardiores.2007.08.008

Scopus ID

2-s2.0-38849208460 (requires institutional sign-in at Scopus site)   101 Citations

Abstract

AIM: Damage to the mitochondrial electron transport chain (ETC) occurs during ischaemia. Blockade of electron flow in the ETC just before ischaemia with the reversible complex I inhibitor amobarbital protects isolated mitochondria against ischaemic damage and preserves oxidative phosphorylation and cytochrome c content. We hypothesized that brief amobarbital perfusion just before ischaemia would improve cardiac recovery and decrease infarct size after ischaemia and reperfusion (IR) by preserving the mitochondrial redox state and reducing mitochondrial superoxide (O(2)(-*)) generation, in turn would decrease mitochondrial Ca(2+) accumulation (mt[Ca(2+)]).

METHODS: Guinea pig Langendorff-perfused hearts were treated with Krebs Ringer solution (KR; untreated) or amobarbital (2.5 mM) in KR for 1 min immediately before 30 min of no flow, global ischaemia, followed by reperfusion without additional treatment. Cardiac function, mitochondrial NADH, FAD, mt[Ca(2+)], and O(2)(-*) levels were assessed during the 1 min perfusion period and throughout IR.

RESULTS: Amobarbital perfusion alone before ischaemia significantly increased O(2)(-*) levels and NADH, without altering FAD, and decreased mt[Ca(2+)]. During ischaemia, mitochondrial NADH was higher, O(2)(-*) levels were lower, and mt[Ca(2+)] was less elevated in the amobarbital group. On reperfusion O(2)(-*) levels and mt[Ca(2+)] were significantly reduced, NADH-FAD redox state was preserved and cardiac function was markedly improved in the amobarbital group; infarct size was smaller in the amobarbital group compared to the untreated group.

CONCLUSION: Temporary blockade of mitochondrial complex I activity by amobarbital protects hearts by reducing production of O(2)(-*) and mtCa(2+) loading during IR injury.

Author List

Aldakkak M, Stowe DF, Chen Q, Lesnefsky EJ, Camara AK

Authors

Amadou K. Camara PhD Professor in the Anesthesiology department at Medical College of Wisconsin
David F. Stowe MD, PhD Professor in the Anesthesiology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Amobarbital
Animals
Calcium
Electron Transport Complex I
Flavin-Adenine Dinucleotide
Guinea Pigs
Myocardial Ischemia
NADP
Oxidation-Reduction
Oxygen
Reactive Oxygen Species
Superoxides
Ventricular Function, Left