Faculty Collaboration Database

Functional expression of transcobalamin II cDNA in Xenopus laevis oocytes. Biochem Biophys Res Commun 1991 Dec 31;181(3):1151-5

Date

12/31/1991

Pubmed ID

1764066

DOI

10.1016/0006-291x(91)92059-s

Scopus ID

2-s2.0-0026357431 (requires institutional sign-in at Scopus site)   8 Citations

Abstract

The products of in vitro transcription of human transcobalamin II (TC II) cDNA when microinjected into Xenopus laevis oocytes yielded a single secretory protein of 43 kDa. The mobility of the 43 kDa band did not change following digestion with peptide N-glycosidase F. [57Co]Cbl bound to the medium was immunoprecipitated with anti-serum to human TC II, but not to other Cbl binders. In addition, the [57Co]Cbl complex also bound to placental microsomes. These results suggest that TC II mRNA transcribed encodes TC II which contains both the Cbl and receptor binding domains. Furthermore, Xenopus oocytes can be used as a screening system to define structural elements important in TC II's secretion and binding reactions.

Author List

Seetharam S, Dahms N, Li N, Seetharam B

Author

Nancy M. Dahms PhD Professor in the Biochemistry department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Cloning, Molecular
DNA
Female
Gene Expression
Humans
Methionine
Oocytes
RNA, Messenger
Recombinant Proteins
Transcobalamins
Transcription, Genetic
Vitamin B 12
Xenopus laevis