Intracellular trafficking pathway of BK Virus in human renal proximal tubular epithelial cells. Virology 2008 Feb 20;371(2):336-49
Date
11/03/2007Pubmed ID
17976677Pubmed Central ID
PMC2674336DOI
10.1016/j.virol.2007.09.030Scopus ID
2-s2.0-38649102147 (requires institutional sign-in at Scopus site) 34 CitationsAbstract
Intracellular trafficking of BK Virus (BKV) in human renal proximal tubular epithelial cells (HRPTEC) is critical for BKV nephritis. However, the major trafficking components utilized by BKV remain unknown. Coincubation of HRPTEC with BKV and microtubule disrupting agents prevented BKV infection as detected by immunofluorescence and western blot analysis with antibodies which recognize BKV large T antigen. However, inhibition of a dynein, cellular motor protein, did not interfere with BKV infection in HRPTEC. A colocalization study of BKV with the markers of the endoplasmic reticulum (ER) and the Golgi apparatus (GA), indicated that BKV reached the ER from 6 to 10 h, while bypassing the GA or passing through the GA too transiently to be detected. This study contributes to the understanding of mechanisms of intracellular trafficking used by BKV in the infection of HRPTEC.
Author List
Moriyama T, Sorokin AAuthor
Andrey Sorokin PhD Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
BK VirusCells, Cultured
Endoplasmic Reticulum
Epithelial Cells
Golgi Apparatus
Humans
Kidney Tubules, Proximal
Kinetics
Microtubules
Time Factors
Virus Internalization