Faculty Collaboration Database

Differential expression of cSmad1 and cSmad5 in the primitive streak during chick embryo gastrulation. Anat Rec 2000 Sep 01;260(1):102-5

Date

09/01/2000

Pubmed ID

10967541

DOI

10.1002/1097-0185(20000901)260:1<102::AID-AR110>3.0.CO;2-1

Scopus ID

2-s2.0-0034282940 (requires institutional sign-in at Scopus site)   3 Citations

Abstract

Whole mount in situ hybridization was performed to determine the expression patterns of cSmad1 and cSmad5 during gastrulation stages (Hamburger-Hamilton 2-6) of chicken embryogenesis. It was revealed that cSmads 1 and 5 mRNAs are prominently expressed in the developing primitive streak. As determined from cross-sectional analysis of stage 4 embryos, most transcripts were associated with the ingressing mesoderm. However, whereas cSmad5 was expressed throughout the streak at all stages examined, the strongest expression of cSmad1 was confined to the posterior half of the streak.

Author List

Gont L, Lough J

Author

John W. Lough PhD Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Chick Embryo
DNA-Binding Proteins
Gastrula
In Situ Hybridization
Mesoderm
Phosphoproteins
RNA, Messenger
Smad Proteins
Smad5 Protein
Tissue Distribution
Trans-Activators