Faculty Collaboration Database

Myosin Id is required for planar cell polarity in ciliated tracheal and ependymal epithelial cells. Cytoskeleton (Hoboken) 2015 Oct;72(10):503-16

Date

10/09/2015

Pubmed ID

26446290

Pubmed Central ID

PMC4715625

DOI

10.1002/cm.21259

Scopus ID

2-s2.0-84954392734 (requires institutional sign-in at Scopus site)   28 Citations

Abstract

In wild type (WT) tracheal epithelial cells, ciliary basal bodies are oriented such that all cilia on the cell surface beat in the same upward direction. This precise alignment of basal bodies and, as a result, the ciliary axoneme, is termed rotational planar cell polarity (PCP). Rotational PCP in the multi-ciliated epithelial cells of the trachea is perturbed in rats lacking myosin Id (Myo1d). Myo1d is localized in the F-actin and basal body rich subapical cortex of the ciliated tracheal epithelial cell. Scanning and transmission electron microscopy of Myo1d knock out (KO) trachea revealed that the unidirectional bending pattern is disrupted. Instead, cilia splay out in a disordered, often radial pattern. Measurement of the alignment axis of the central pair axonemal microtubules was much more variable in the KO, another indicator that rotational PCP is perturbed. The asymmetric localization of the PCP core protein Vangl1 is lost. Both the velocity and linearity of cilia-driven movement of beads above the tracheal mucosal surface was impaired in the Myo1d KO. Multi-ciliated brain ependymal epithelial cells exhibit a second form of PCP termed translational PCP in which basal bodies and attached cilia are clustered at the anterior side of the cell. The precise asymmetric clustering of cilia is disrupted in the ependymal cells of the Myo1d KO rat. While basal body clustering is maintained, left-right positioning of the clusters is lost.

Author List

Hegan PS, Ostertag E, Geurts AM, Mooseker MS

Author

Aron Geurts PhD Professor in the Physiology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Actins
Animals
Animals, Genetically Modified
Axoneme
Basal Bodies
Brain
Cell Polarity
Cilia
Ependyma
Epithelial Cells
In Situ Nick-End Labeling
Intestines
Male
Mice, Knockout
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Microscopy, Fluorescence
Myosin Type I
Myosins
Neuroglia
Phenotype
Rats
Rats, Inbred F344
Rotation
Trachea